Tuesday 14th of June 2011 |
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| 08:30 |
Registration |
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Symposium hall A |
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| 09:30 |
Welcome and opening of symposium |
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Plenary speaker |
| 09:45 |
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The evolution of PCR-based assays: from fully manual to fully automated
Russell Higuchi, PhD, Cepheid Fellow, R&D, Sunnyvale, CA, US.
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Preanalytics and standardization session |
| 10:15 |
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Generic Pre-analytical Workflows: Challenges and Solutions
Uwe Oelmueller, PhD, Vice president R&D Diagnostic Sample Preparation & Stabilization, QIAGEN GmbH. Hilden, Germany
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| 10:40 |
Break, coffee and tea in the exhibition area |
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| 11:10 |
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"Translational Standardization": The challenge of finding, applying and adapting appropriate standardization resources throughout the processes of translational research – to clinical implementation
Roberta
Madej, Executive Director, Technical Support- Biologics, Cepheid, and Chair,
Consensus Committee on Molecular Methods, CLSI, USA
 Pdf
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| 11:35 |
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MIQE:
quality control and standardisation for real-time PCR
Stephen A. Bustin, Professor of Molecular Science, Institute of Cell and Molecular Science, Queen Mary's School of Medicine and Dentistry, University of London, UK
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| 12:00 |
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RTq-PCR and Microarray Analysis of Fluorescence Activated Cell Sorter-derived Cells: Creating Harmony between Technologies
Scott Tighe, University of Vermont, Advanced Genome Technology Core, Burlington, VT, USA.
Pdf
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| 12:25 |
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Olink short presentation
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| 12:30 |
Lunch |
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| 13:40 |
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The pre-analytical phase for molecular methods in Blood Samples
Mario Pazzagli, Professor of Clinical Biochemistry and Clinical Molecular Biology, University of Florence, Italy
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| 14:05 |
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The Impact of Tissue Pre-Analytics on Molecular Analyses
Christian Viertler, MD, Institute of Pathology, Medical University of Graz, Austria
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| 14:30 |
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How to work with biobanked samples, the possibilities and impossibilities
Marcel Kap, Dept of Pathology, Josephine Nefkens Institute, Erasmus Medical Center, Rotterdam, The Netherlands
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| 14:55 |
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TIBmolbiol short presentation
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| 15:00 |
Break, coffee and tea in the exhibition area |
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| 15:30 |
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Preparation of mRNA directly, a stable and less labour intensive way to prepare samples for expression analysis
Kerstin Ekeland-Sjöberg, Genetic Analyses, Laboratory of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden
Pdf
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| 15:55 |
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ValidPrime for accurate real time qPCR quantification of RNA without DNase treatment
Henrik Laurell, PhD, Inserm, Toulouse, France
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| 16:20 |
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Problems with contaminating DNA? Different sources – different strategies for removal.
Morten Elde, PhD, Head of Application Development, ArcticZymes AS, Norway
Pdf
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| 16:45 |
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Sealing choices in qPCR
Simon Baker, Oxford Brookes University, UK
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17:10
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Panel Discussion
Moderator: Mikael Kubista
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18:10-
19:00 |
Poster session, mingel and networking
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| 20:00 |
Dinner in the city of Prague, Restaurant Vikarka |
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Tuesday 14th of June |
Symposium hall B |
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Session: New applications and technologies |
| 13:40 |
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The use of qRT-PCR and high-throughput transcriptomics for biomarker development
Michael W. Pfaffl, Professor of Molecular Physiology, Technical University of Munich, Weihenstephan, Germany
Pdf
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| 14:05 |
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qPCR - an indispensable Tool for DNA Methylation Biomarker Validation
Andreas Weinhäusel, Health & Environment Department, Molecular Medicine, AIT Austrian Institute of Technology, Vienna, Austria
Pdf
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| 14:30 |
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Customized, function tested
RealTime ready assays for gene expression analysis in biomarker research and
early drug development
Sabine Lohmann, PhD, Roche Diagnostics GmbH, Penzberg, Germany
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| 14:55 |
Break, coffee and tea in the exhibition area |
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| 15:30 |
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The patenting of PCR and qPCR in the EPO
Hans-Rainer Jaenichen, PhD, European Patent Attorney, Vossius & Partner, Munich, Germany
Pdf
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| 15:55 |
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Development of ultra small real-time PCR system
Pavel Neuzil, KIST-Europe, Germany
Pdf
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| 16:20 |
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Isothermal real-time RNA
amplification in a miniaturised platform for environmental monitoring of toxic
algal blooms
Maria-Nefeli Tsaloglou, PhD, National Oceanography Centre
Southampton, Hampshire, UK
Pdf
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16:45-
17:10
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A new generation of qPCR assays which use internally quenched probes
Mark Behlke MD, PhD, Chief Scientific Officer, Integrated DNA Technologies, Coralville, USA
Pdf
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Wednesday 15th of June 2011 |
Symposium hall A |
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Session: Circulating tumor cells (CTC) |
| 09:00 |
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Circulating tumor cells: Challenges & Perspectives
Klaus Pantel, Direktor, Institute of Tumorbiology, University Medical Center Hamburg-Eppendorf, Germany
Pdf
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| 09:30 |
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Detection and characterization of single circulating tumor cells as a chance to monitor and individualize chemotherapy
Katharina Pachmann, MD, PhD, Department for Experimental Haematology und Oncology Clinic for Internal Medicine II Friedrich Schiller University Jena, Germany
Pdf
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| 09:55 |
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Challenges of new discoveries; clinical applications into the management of cancer patients
Tomáš Zima, Head Institut of Clinical Chemistry and Laboratory Medicine of the First Faculty of Medicine and General Teaching Hospital, Prague, Czech Republic
Pdf
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| 10:20 |
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Highly sensitive and specific detection of cancer mutations using
myT™ primer qPCR
Vladimir
Makarov, PhD, Chief Scientific Officer, Swift
Biosciences, Ann Arbor, USA
Pdf
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10:45
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Lonza short presentation
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10:50
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Break, coffee and tea in the exhibition area |
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Session: MOlecular Diagnostics |
11:10
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Macula Risk ® - A Prognostic Test for Age-related
Macular Degeneration (AMD)
Preveen Ramamoorthy, PhD, Director, Molecular Diagnostics, Advanced Diagnostic Laboratories, Assistant Professor, Department of Medicine, National Jewish Health, USA
Pdf
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| 11:35 |
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Minimizing variation in
gene expression analysis when using real-time PCR
Ian
Kavanagh PhD, Senior R&D
Manager, Thermo
Scientific, Genomics, England
Pdf
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| 12:00 |
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Use
of a designer Taq mutant for rapid qPCR
David
Sugden, qStandard, U.K
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12:25
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KBioscience short presentation
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| 12:30 |
Lunch |
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Session: microRNA |
| 13:30 |
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miQPCR: A novel method to profile
microRNAs by quantitative real-time PCR
Mirco Castoldi, PhD, EMBL, Heidelberg, Germany
Pdf
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| 13:55 |
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| 14:20 |
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Altered expression of microRNAs in human cancer: the influence of genetic and epigenetic factors
Claudio Orlando, PhD, Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Italy
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| 14:45 |
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An Extremely Simple and Sensitive Micro RNA Detection and Profiling Method
Tom Xu, PhD, Principal Scientist, Applied Biosystmes, Life Technologies Corporation, USA
Pdf
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| 15:10 |
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| 15:15 |
Break, coffee and tea in the exhibition area |
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Session: Single cell qPCR |
| 15:45 |
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Single cell expression profiling
Mikael Kubista, Prof. Institute of Biotechnology, CAS, and founder of TATAA Biocenter
Pdf
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| 16:10 |
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Single-cell gene
expression profiling: High throughput analysis and digital PCR
Anders Ståhlberg, PhD, Cancer center, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden
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| 16:35 |
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Interpreting heterogeneous transcript expression across populations of single leukaemic cells
Philip Day, PhD, Reader in Quantitative Analytical Genomics, The Manchester Interdisciplinary Biocentre, University of Manchester, UK
Pdf
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17:00-
17:10 |
Closing of Symposium |
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Wednesday 15th of June 2011 |
Symposium hall B |
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Session: Data analysis and normalization |
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| 09:00 |
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Error in qPCR quantification
Ales Tichopad, PhD, TATAA Molecular Diagnostics, Prague, Czech Republic
Pdf
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09:30
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Hypothesis-Driven
Approaches to Multivariate Analysis of qPCR Data
Anders Bergkvist, PhD, Application Specialist,
Sigma-Aldrich, Sweden
Pdf
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09:55
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Selecting suitable reference genes for qPCR analyses of auto-immune disease blood samples
Oana Popa-Nita,Project Manager Auto-Immune Diseases, TcLand Expression, Nantes, France
Pdf
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10:20
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An Internal Control for monitoring DNA extraction and PCR inhibition in Real-Time PCR assays
Steve Hawkins, Bioline, London, UK
Pdf
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10:45
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Analytic Jena short presentation
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10:50
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Break, coffee and tea in the exhibition area |
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Session: Circulating tumor cells (CTC) |
| 11:10 |
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mRNA and microRNA expression profiling of circulating tumor cells (CTCs)
Anieta M. Sieuwerts, PhD, Erasmus Medical Center, Dept of Translational Cancer Genomics and Proteomics Rotterdam, The Netherlands
Pdf
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11:35
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Circulating Tumor Cells and molecular Biomarkers in Melanoma
Pamela Pinzani, PhD, Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Italy
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12:00
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Comparative
study of circulating tumor cells (CTCs) versus primary tumors using ScreenCell
technology, a rapid and save method of CTC selection based on size
Janine Wechsler, PhD MD, Consultant Pathologist, ScreenCell Company, Paris, France
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| 12:30 |
Lunch |
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| 13:30 |
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Breast cancer, what do CTCs mean?
Michel Rigaud, Prof. University of Limoges, Limoges, France
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| 13:55 |
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Circulating tumor cells - a
new tool for optimizing diagnosis and treatment of primary breast cancer
patients
Tanja Fehm, Prof. Dr., Dept. Gyn/OB, Tuebingen, Germany
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14:20-
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Characteristics
and prognostic value of circulating tumor cells in blood of metastatic breast
cancer patients
Sabine Kasimir-Bauer, Prof. Dr. rer.nat., Klinik für Frauenheilkunde und Geburtshilfe, Universitätsklinikum Essen, Germany
Pdf
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